Devolepment of an Enhanced Immuno-dot Blot Assay to Detect White Spot Syndrome Virus in Shrimp Using Antobody Conjugated Gold Nanoparticle Probe
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چکیده
White Spot Syndrome Virus (WSSV) is a double-stranded (305 kb) DNA invertebrate virus (Wang et al 1998) that causes White Spot Syndrome (WSS) in almost all commercially available crustaceans. Though 22 different viruses are known to cause diseases in shrimp (Hsu et al 2000), WSSV is particularly of great interest to cause mass mortality in 3-10 days of disease blitz. The estimated annual global economic losses due to WSSV are close to three billion dollars (Lundin et al 1996).
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Design and Evaluation of a PCR Method for Detecting White Spot Syndrome Virus in Shrimp
Background: White spot syndrome virus (wssv) is the causing agent for white spot disease in shrimp and many crustaceans. This disease is highly contagious and can cause death within 3–10 days under normal culture conditions. Therefore, early diagnosis of the virus is a necessity. Materials and Methods: Primers were designed for three regions of the virus genome and one region of the shrimp geno...
متن کاملImmunomodulatory effects of seagrass Halophila ovalis polysaccharide mixed feed in adult black tiger shrimp Penaeus monodon and its protective efficacy against white spot syndrome virus infection
As white spot syndrome virus (WSSV) can be highly pathogenic in penaeid shrimp, various feed supplements have been tested to help to protect farmed shrimp against WSSV disease. Here a polysaccharide extract from Halophila ovalis (HO) seagrass was added to feeds at concentrations of 0.25, 0.5, and 1.0 g[a1] /kg to assess its ability to protect Black Tiger shrimp (Penaeus monodon) against WSSV ch...
متن کاملThe Status of white spot syndrome virus (WSSV) in Islamic Republic of Iran
White spot syndrome virus (WSSV), the causative virus of disease, is found in most shrimp farming areas in Iran, and it cause large economic losses to the shrimp farming industry. Shrimp aquaculture is an important industry in Iran and plays an important role in aquaculture production. The shrimp production in 2012 was more than 12000 metric tonnes and it is estimated it will reach 20000 tonnes...
متن کاملThe Status of white spot syndrome virus (WSSV) in Islamic Republic of Iran
White spot syndrome virus (WSSV), the causative virus of disease, is found in most shrimp farming areas in Iran, and it cause large economic losses to the shrimp farming industry. Shrimp aquaculture is an important industry in Iran and plays an important role in aquaculture production. The shrimp production in 2012 was more than 12000 metric tonnes and it is estimated it will reach 20000 tonnes...
متن کاملImmunomodulatory effects of seagrass Halophila ovalis polysaccharide mixed feed in adult black tiger shrimp Penaeus monodon and its protective efficacy against white spot syndrome virus infection
As white spot syndrome virus (WSSV) can be highly pathogenic in penaeid shrimp, various feed supplements have been tested to help to protect farmed shrimp against WSSV disease. Here a polysaccharide extract from Halophila ovalis (HO) seagrass was added to feeds at concentrations of 0.25, 0.5, and 1.0 g[a1] /kg to assess its ability to protect Black Tiger shrimp (Penaeus monodon) against WSSV ch...
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